Solubilization and partial purification of UDP‐galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 401-406
- https://doi.org/10.1016/0014-5793(86)81057-2
Abstract
We have developed procedures to solubilize the envelope UDP‐galactose:diacylglycerol galactosyltransferase activity and to assay this enzyme after solubilization with a zwitterionic non‐denaturing detergent (CHAPS) and fractionation. From solubilized envelope membranes isolated from intact spinach chloroplasts, we were able to prepare by chromatography on hydroxyapatite a fraction enriched (6–7‐fold) in this enzyme responsible for monogalactosyldiacylglycerol synthesis.Keywords
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