Structural characterization of the lipopolysaccharide O‐antigen and capsular polysaccharide of Vibrio ordalii serotype O : 2

Abstract
Structures of the capsular and O‐chain polysaccharides of Vibrio ordalii serotype O : 2, the causative agent of vibriosis in salmonid fish, were determined by high‐field NMR techniques, mass spectrometric methods and partial hydrolysis. Both polymers were shown to be composed of linear tetrasaccharide repeating units, having the structure : [←4)‐β‐ D‐Glcp2NAc3NAN‐(1←4)‐β‐Glcp2NAc3NAmA‐(1←4)‐α‐ L‐Galp2NAc3NAcA‐(1←3)‐β‐ D‐Sugp‐(1←]n, ∣ Fo‐ L‐Ala where Am = acetamidino; Fo = formyl; AN = amide, and Sug = 2‐acetamido‐2,6‐dideoxy‐ D‐xylo‐hexos‐4‐ulose.

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