Lack of Genotoxic Effects of Fluoride in the Mouse Bone-marrow Micronucleus Test

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Abstract
The purpose of this investigation was to examine the potential genotoxic influence of sodium fluoride (NaF) on mammalian cells by means of a mouse bone-marrow micronucleus test. Mice of genotype B6C3F1 were obtained at about eight weeks of age and maintained on a low-fluoride diet (< 0.2 ppm F) and distilled water ad libitum throughout the experiment. At approximately 12 weeks of age, the animals were randomly assigned to seven groups, with multiple sampling schedules. The animals were intubated with various doses of NaF, ranging from 0.1 mg/kg to the Maximum Tolerable Dose (MTD), with sampling at 30, 48, and 72 hours after treatment. Negative (distilled water) and positive (cyclophosphamide) controls were included. Coded slides of femur marrow cells were prepared and examined, without identification as to treatments, for the frequency of micronucleated polychromatic erythrocytes (MN-PCE). The humera were analyzed for fluoride for monitoring of the absorption of fluoride following oral intubation. The results indicated that doses of NaF up to the MTD did not significantly increase the frequencies of MN-PCE when compared with the negative controls, although the bone fluoride content increased as the dose of NaF was increased.

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