In vivo promoter activity of the synthetic Pribnow box
- 28 January 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 278 (2) , 207-210
- https://doi.org/10.1016/0014-5793(91)80118-m
Abstract
The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA), ??? a containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been show that these inserts are able to induced an efficient in vivo expression of the gal operon.This publication has 9 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Transcriptionin vivodirected by consensus sequences ofE.colipromoters: their context heavily affects efficiencies and start sitesNucleic Acids Research, 1990
- In vivogene expression directed by synthetic promoter constructions restricted to the −10 and −35 consensus hexamers ofE.coliNucleic Acids Research, 1989
- DNA sequences of random origin as probes of Escherichia coli promoter architecture.Journal of Biological Chemistry, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Analysis of E.Coli Pormoter sequencesNucleic Acids Research, 1987
- Characterization of two mutations in the Escherichia coli galE gene inactivating the second galactose operator and comparative studies of repressor binding.The EMBO Journal, 1983
- Synthesis of deoxyoligonucleotides on a polymer supportJournal of the American Chemical Society, 1981
- The nucleotide sequence of the initiation and termination sites for ribosomal rna transcription in x. laevisCell, 1979