MSSP, a protein binding to an origin of replication in the c‐myc gene, interacts with a catalytic subunit of DNA polymerase α and stimulates its polymerase activity

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Abstract
MSSP has been identified as a protein that binds to both single‐ and double‐stranded sequences of a putative DNA replication origin sequence in the human c‐myc gene. MSSP possesses versatile functions, including stimulation of DNA replication, transcriptional regulation, apoptosis induction, and cell transformation coordinated by c‐Myc. MSSP contains two RNP domains, RNP1‐A and RNP1‐B, both of which are necessary for all of the functions of MSSP. In this study, we found that MSSP binds to the N‐terminal region of a catalytic subunit of a human DNA polymerase α via its RNP domains both in vitro and in human cells. Furthermore, MSSP was released from the putative DNA replication origin of the c‐myc gene after it complexed with DNA polymerase α, and MSSP stimulated DNA polymerase activity in vitro.