Determination of copper-oxinate in plant tissues by high performance liquid chromatography using fluorimetric detector.

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Abstract
A high performance liquid chromatography method for the determination of copperoxinate in plant tissues was developed. The method involves isolation of the residue by extraction with acetone, decomposition of extracted residue to 8-hydroxyquinoline by masking copper (II) ion with potassium cyanide followed by high performance liquid chromatography with a fluorimetric detector. Non-fluorescent 8-hydroxyquinoline separated from copper-oxinate was converted to a highly fluorescent chelate and eluted from the column by the eluent containing Al (III) ion. The lowest limit of detection and recoveries from plant tissues sample were about 0.01 ppm and 86.1-103.1%, respectively.

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