Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product
- 1 October 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 287 (5785) , 801-805
- https://doi.org/10.1038/287801a0
Abstract
We previously determined the nucleotide sequence of the 3′ end of Moloney leukaemia virus and discovered the potential coding region for an unknown protein, R. We now show that this region does encode a protein. A pentadecapeptide of R was chemically synthesized and antibodies raised against it. Antisera to the synthetic peptide recognize the R protein and the env precursor polyprotein in infected cells. The strategy presented here should provide a general method for accessing proteins predicted by nucleotide sequences.Keywords
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