Occurrence of two distinct succinate thiokinases in animal tissues
Open Access
- 7 April 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 199 (1) , 57-60
- https://doi.org/10.1016/0014-5793(86)81223-6
Abstract
Although succinate thiokinase from mammalian sources has hitherto been described as showing substrate specificity for guanine nucleotide, a range of mammalian tissues has here been found to display succinate thiokinase activity with both guanine and adenine nucleotides as substrates. Evidence is presented for the existence of two distinct succinate thiokinases and this is confirmed by their separation by affinity chromatography. Each enzyme is specific for one nucleotide and is inhibited by the non‐substrate nucleotide. The physiological roles of the two enzymes is yet to be established.Keywords
This publication has 13 references indexed in Scilit:
- Patterns of nucleotide utilisation in bacterial succinate thiokinasesFEBS Letters, 1982
- An ATP-dependent succinic thiokinase in birds and its relation to ketone-body utilizationFEBS Letters, 1981
- Affinity chromatography and affinity labeling of rat liver succinyl-CoA synthetase.Journal of Biological Chemistry, 1980
- Succinate thiokinase from cyanobacteriaFEBS Letters, 1980
- Effect of environmental oxygen tensions on in vivo intestinal absorption of glycine by the rainbow trout (Salmo gairdneri r.)Comparative Biochemistry and Physiology Part A: Physiology, 1979
- [5] Preparation of bovine heart mitochondria in high yieldPublished by Elsevier ,1979
- Occurrence of ‘large’ or ‘small’ forms of succinate thiokinase in diverse organismsFEBS Letters, 1978
- An adenine nucleotide‐linked succinic thiokinase of animal originFEBS Letters, 1973
- [56] Polarographic assay for malate synthase and citrate synthasePublished by Elsevier ,1969
- [13] Succinate thiokinase from pig heartPublished by Elsevier ,1969