Purification of Aldolase C from Rat Brain and Hepatoma

Abstract
An isolation procedure for rat brain aldolase C has been developed which also permits the isolation of aldolase C from experimental hepatomas.Certain enzymatic properties (specific activity and Michaelis constant towards the two specific substrates: fructose 1,6‐bisphosphate and fructose 1‐phosphate) and physico‐chemical properties (molecular weight, N‐terminal amino‐acid) of the two enzymes have been studied and compared. Moreover, an amino‐acid analysis has been carried out for rat brain aldolase C.Within experimental errors, the two enzymes appear to be identical.