A rapid and simple procedure for purification of S-II, a transcription factor of ribonucleic acid polymerase II.

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Abstract
We previous reported the purification of eukaryotic transcription fctor S-II and its phosphorylated form S-II'' from Ehrlich ascites tumor cells. In this work, we modified the previous purification procedure to make it quicker and simpler. By this improved procedure, involving a different solubilization method, and improved buffer solutions and centrifugation processes, S-II could be purified to homogeneity for 1 kg of frozen cells in about 10 d instead of 1 month. The purity, specific activity and total yield of S-II purified by the new method were comparable to those obtained by using the previous method.