A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13.
Open Access
- 1 April 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 6 (4) , 314-326
- https://doi.org/10.1101/gr.6.4.314
Abstract
The genomic sequence of the human CD4 gene and its neighboring region, located at chromosome 12p13, was generated using the large-scale shotgun sequencing strategy. A total of 117 kb of genomic sequence and approximately 11 kb of cDNA sequence were obtained. Six genes, including CD4, triosephosphate isomerase, B3 subunit of G proteins (GNB3), and ubiquitin isopeptidase T (ISOT), with known functions, and two new genes with unknown functions were identified. Using a battery of strategies, the exon/intron boundaries, splice variants, and tissue expression patterns of the genes were determined. Various computer software was utilized for analyses of the DNA and amino acid sequences. The results of the analyses and sequence-based strategies for gene identification are discussed.Keywords
This publication has 33 references indexed in Scilit:
- Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase TBiochemistry, 1995
- Sequencher for the Macintosh (Version 3.0, March, 1995)produced by Gene Codes. $2050.00 (academic)/$2850.00 (commercial); includes all updates and technical support for one year after releaseTrends in Biochemical Sciences, 1995
- A lineage-specific transcriptional silencer regulates CD4 gene expression during T lymphocyte developmentCell, 1994
- The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogeneNature, 1993
- A human dimorphism resulting from loss of an AluGenomics, 1992
- Isolation and characterization of a cDNA encoding a synaptonemal complex proteinBiochemistry and Cell Biology, 1992
- The T Cell Receptor as a Multicomponent Signalling Machine: CD4/CD8 Coreceptors and CD45 in T Cell ActivationAnnual Review of Immunology, 1992
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAVNature, 1984
- The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirusNature, 1984