Antigenic Analysis of Chlamydiae by Two-Dimensional Immunoelectrophoresis
Open Access
- 1 October 1975
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 115 (4) , 963-968
- https://doi.org/10.4049/jimmunol.115.4.963
Abstract
Two-dimensional immunoelectrophoresis was used for the antigenic analysis of Chlamydia. Antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent Triton X-100. Nineteen and 16 distinct components were observed for Chlamydia trachomatis strain L2/434/Bu and C. psittaci strain Meningopneumonitis/Cal-10, respectively. The triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed between 434 and Cal-10. However, the antigens of Trachoma strain K/UW-31/Cx and LGV strain 434 were found to be very similar.Keywords
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