Unzipping Kinetics of Double-Stranded DNA in a Nanopore

Preprint

2 May 2003

preprint
Published by arXiv in arXiv

https://doi.org/10.48550/arXiv.cond-mat/0209414

Abstract

We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.

Keywords

UNZIPPING KINETICS
SINGLE MOLECULES
NARROW PROTEIN
DNA UNZIPPING
STRANDED DNA
DIRECT PROOF
TRAVERSAL

All Related Versions

Version 1, 2002-09-17, ArXiv
Version 2, 2003-03-26, ArXiv
Version 3, 2003-05-02, ArXiv
Published version: Physical Review Letters, 90 (23), 238101.

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