Bound Water and Frost Hardiness.

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Abstract
Leaves of hardened cabbage dried at 0% relative humidity and 3[degree]C retained larger amounts of bound water removable at 100[degree]C than leaves of unhardened cabbage. Both leaf extract and leaf residues showed the same differences as the whole leaf. The mixture of mitochondria and chloroplast grana, the whole chloroplasts, and the grana all showed the above differences; the cytoplasmic proteins and the mixture of microsomes and grana did not. The method adopted for measuring "bound water" therefore succeeded in showing that at least some protoplasmic components undergo an increase in hydration capacity during the hardening period.