The Age-Dependent Cytokine Production by Murine CD8+ T Cells as Determined by Four-Color Flow Cytometry Analysis
Open Access
- 1 March 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journals of Gerontology: Series A
- Vol. 54 (3) , B116-B123
- https://doi.org/10.1093/gerona/54.3.b116
Abstract
Flow cytometry has been used to simultaneously examine intracellular cytokine production and expression of CD44 and CD45RB by murine CD8+ T cells derived from young (2–3 months) or aged (18–22 months) mice. Cytokine production by aged CD8+ T cells differs from that by CD8+ T cells derived from young animals in that a significantly higher percentage of the aged can be triggered to produce interleukin (IL)-4, interferon (IFN)-γ, and tumor necrosis factor α (TNFα). Conversely, a greater fraction of young CD8+ T cells produce IL-2. Aged mice not only have a higher percentage of CD8+/CD44hi T cells, but also a larger fraction of these cells are IFN-γ+ and IL-4+, while a lower fraction are IL-2+ than is observed in young CD8+/CD44hi T cells. In terms of relative contribution to total cytokine synthesis, a greater fraction of CD8+ T cells produce IFN-+ and IL-4 than in CD4+ T cells, whereas CD4+ T cells are the major producers of IL-2.Keywords
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