Immunologic Studies of Glycoproteins Isolated From Cell Membranes of Human Lung Carcinomas2

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Abstract
Lithium diiodosalicylate was used to isolate glycoproteins from microsomal preparations of human lung carcinomas and from normal human lung tissue. A glycoprotein present in the extracts of all but 1 of the oat-cell lung carcinomas was identical to carcinoembryonic antigen (CEA) by Ouchterlony immunodiffusion analysis. Extracts of epidermoid lung carcinomas contained antigens either identical to, or cross-reacting with, CEA by immuno-diffusion analysis, and extracts from 2 lung adenocarcinomas had antigens that cross-reacted with CEA. An antigen in the glycoprotein extract of lung tissue from 2 noncancer patients also cross-reacted with CEA. Although CEA appeared to have at least 1 antigenic determinant not present in the cross-reacting glycoproteins from some epidermoid carcinomas, adenocarcinomas, and normal lung, glycoproteins from the lung tumors and normal lung did not have any detectable antigenic determinants not found on CEA. No membrane glycoprotein antigens specific for lung cancer were identified.

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