Retention Behavior of Benzodiazepines in Normal-Phase HPLC. Silica, Cyano, and Amino Phases Comparison

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Abstract
The retention data for 16 benzodiazepines was evaluated on different normal-phase HPLC columns using the Soczewinski-Snyder model. The slopes and intercepts of the linear relationships between log k' and the mobile phase composition have been discussed in relation to solute and stationary phase characteristics. The good correlation between substituent chromatographic contribution and substituent electronic constant showed that the benzodiazepine molecule acts as a proton donor towards the adsorbent sites of the stationary phases investigated.