Binding Characteristics of Human Serum Amyloid P Component
- 1 March 1987
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 25 (3) , 275-281
- https://doi.org/10.1111/j.1365-3083.1987.tb01073.x
Abstract
Serum amyloid P component (SAP), a normal human plasma glycoprotein, was found in a solid phase ELISA to have Ca2+-dependent binding for keyhole limpet haemocyanin (KLH), pectic acid, trinitrophenylated (TPN) macromolecules, and plastic surfaces. The binding to (TNP)-KLH was used to develop a sensitive ELISA. The binding of SAP to the ligands mentioned was inhibited by EDTA, KLH, pectic acid, TNP-conjugated macromolecules (bovine serum albumin, polyacrylhydrazide), and p-nitrophenylarsonic acid. Underivatized and DNP-conjugated macromolecules did not inhibit the SAP binding; arsenilic acid, picric acid, and dinitrophenyl were weak inhibitors. SAP bound to TNP-agarose was eluated by either EDTA or p-nitrophenylarsonic acid. Thus, a unique region of SAP is responsible for the polyspecific binding. We suggest that the polyspecific binding of SAP takes place through a Ca2+ bridge; half of the metal coordination sphere is occupied by SAP, with the other half available to interact with metal ligand.This publication has 16 references indexed in Scilit:
- Human serum amyloid P component, a circulating lectin with specificity for the cyclic 4,6-pyruvate acetal of galactose. Interactions with various bacteriaBiochemical Journal, 1985
- Binding specificity of serum amyloid P component for the pyruvate acetal of galactose.The Journal of Experimental Medicine, 1984
- LARGE SCALE ISOLATION AND PARTIAL PRIMARY STRUCTURE OF HUMAN PLASMA AMYLOID P‐COMPONENT*Annals of the New York Academy of Sciences, 1982
- Isolation of human C-reactive protein and serum amyloid P componentJournal of Immunological Methods, 1982
- Fibronectin and C4-binding protein are selectively bound by aggregated amyloid P componentThe Journal of Experimental Medicine, 1981
- The Amyloid P‐Component (Protein AP): an Integral Part of the Amyloid Substance?Scandinavian Journal of Immunology, 1979
- Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins).Proceedings of the National Academy of Sciences, 1977
- A simplified method for cyanogen bromide activation of agarose for affinity chromatographyAnalytical Biochemistry, 1974
- Humanserumproteine mit hoher Affinität zu Carboxymethyl- Cellulose, III. Physikalisch-chemische und immunologische Charakterisierung eines metallbindenden 9,5S-αi-Glykoproteins (CM-Protein III)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1972
- Basic Exercises in ImmunochemistryPublished by Springer Nature ,1969