STUDIES ON CHEMOTAXIS .2. SIGNIFICANCE OF NORMAL SERA FOR CHEMOTAXIS INDUCED BY VARIOUS AGENTS

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  • 1 January 1965
    • journal article
    • research article
    • Vol. 9  (5) , 441-+
Abstract
Fresh normal sera of rabbits, guinea pigs, pigs, calves, sheep and men when incubated with complexes of human serum albumin and rabbit antibody induce chemotaxis of rabbit granulocytes in vitro. No chemotaxis was observed when such sera were heated prior to the addition of antigen-antibody complexes. Incubation of fresh normal rabbit serum with heat-aggregated gamma-globulin, PPD, glycogen, Proteus endotoxin or heat-killed Staphylococcus albus results in formation of chemotactic factor(s). No chemotactic activity is found if these agents are incubated in inactivated normal rabbit serum or in serum-free medium. In contrast, the chemotactic activity of Witte''s peptone does not depend on the presence of serum.