SPECIFIC RADIOIMMUNOASSAY OF CYCLO (HIS-PRO), A BIOLOGICALLY ACTIVE METABOLITE OF THYROTROPIN-RELEASING HORMONE
- 1 May 1981
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 108 (5) , 1995-1997
- https://doi.org/10.1210/endo-108-5-1995
Abstract
We have developed a radioimmunoassay that can detect 10 pg cyclo (His-Pro) in rat brain perchloric acid extracts. The normal rat brain concentration of cyclo (His-Pro) ranged from 35–61 pmole/brain. The elution profiles of cyclo (His-Pro)-like immunoreactivity and radioactive cyclo (His-Pro) following gel filtration and ion-exchange chromatography were similar.Keywords
This publication has 5 references indexed in Scilit:
- Cyclo (His-Pro): A selective inhibitor of rat prolactin secretionLife Sciences, 1980
- Histidyl-proline diketopiperazine, an endogenous brain peptide that inhibits (Na+ + K+)-ATPaseBiochemical and Biophysical Research Communications, 1980
- Antagonism of ethanol-induced decrease in rat brain cGMP concentration by histidyl-proline diketopiperazine, a thyrotropin releasing hormone metaboliteBiochemical and Biophysical Research Communications, 1979
- Thermoregulation in rats: Opposing effects of thyrotropin releasing hormone and its metabolite histidyl-proline diketopiperazineBiochemical and Biophysical Research Communications, 1978
- Demonstration of pyroglutamylpeptidase and amidase activities toward thyrotropin-releasing hormone in hamster hypothalamus extracts.Journal of Biological Chemistry, 1976