Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.

Open Access

1 November 1994

journal article
research article
Published by BMJ in Journal of Clinical Pathology

Vol. 47 (11) , 1043-1044
https://doi.org/10.1136/jcp.47.11.1043

Abstract

A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.

Keywords

GENOMIC DNA
PROTEINASE K
EXTRA BURDEN
ETHANOL PRECIPITATION
INVOLVES PROTEINASE
EASILY OBTAINABLE
OBTAINABLE CLOTTED
LABORATORY TESTS
SIMPLE METHOD
K DIGESTION

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Nucleic Acids Research, 1992
PCR based diagnosis in the presence of 8% (v/v) blood
Nucleic Acids Research, 1991
An efficient salt-chloroform extraction of DNA from blood and tissues.
1989