A High-Resolution Radiation Hybrid Map of the Human Genome Draft Sequence
- 16 February 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 291 (5507) , 1298-1302
- https://doi.org/10.1126/science.1057437
Abstract
We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.Keywords
This publication has 22 references indexed in Scilit:
- The Sequence of the Human GenomeScience, 2001
- Integration of cytogenetic landmarks into the draft sequence of the human genomeNature, 2001
- A physical map of the human genomeNature, 2001
- Initial sequencing and analysis of the human genomeNature, 2001
- Multipoint Radiation Hybrid Mapping: Comparison of Methods, Sample Size Requirements, and Optimal Study CharacteristicsGenomics, 1994
- NIGMS Human/Rodent Somatic Cell Hybrid Mapping Panels 1 and 2Genomics, 1993
- Characterization of NIGMS Human/Rodent Somatic Cell Hybrid Mapping Panel 2 by PCRGenomics, 1993
- Radiation Hybrid Mapping: A Somatic Cell Genetic Method for Constructing High-Resolution Maps of Mammalian ChromosomesScience, 1990
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990