Cultivation of an L‐lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle
- 5 June 1994
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 44 (1) , 21-28
- https://doi.org/10.1002/bit.260440105
Abstract
Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD‐15, on sucrose at 70°C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system. © 1994 John Wiley & Sons, Inc.Keywords
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