Relationships Between Genetic Polymorphisms and Anticancer Drug Cytotoxicity Vis-à-Vis the NCI-60 Panel

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Abstract
Introduction: The National Cancer Institute (NCI)-60 panel consists of 60 human tumor cell lines initially established for screening thousands of molecules for antiproliferative activity. It has been powerful for deciphering the relationships between anticancer drug cytotoxicity and cell molecular characteristics. We tested its potential interest for establishing relationships between the polymorphism of genes involved in drug metabolism and transport or in DNA repair, and drug cytotoxicity extracted from NCI databases. Methods: Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques, three frequent single nucleotide polymorphisms (SNPs) were analyzed: Lys751Gln in the Xeroderma pigmentosum complementation group D (XPD, ERCC2) gene, Asp1104His in the Xeroderma pigmentosum complementation group G (XPG, ERCC5) gene and Ile105Val in the glutathione S-transferase P1 (GSTP1) gene. Results: The allelic frequencies of the variants were 33% for ERCC2, 23% for ERCC5 and 39% f...
Funding Information
  • Ligue Nationale contre le Cancer, comités de la Dordogne, de la Charente et de la Charente Maritime
  • National Cancer Institute NCI