Some Effects of Temperature on the Early Stages of Tobacco Necrosis Virus Multiplication

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Abstract
The number of infective centers which were established successfully following the manual inoculation of French bean leaves with tobacco necrosis virus strain D (TNVD) or with TNVD RNA, decreased with increasing temperature between 13-30.degree. C. At 30.degree. C or above, primary and probably also secondary infections could not be established, though it is likely that a limited amount of virus RNA and nucleoprotein was produced at 30.degree. C in cells in which infection was established previously at 23.degree. C. During the 1st day after inoculation, 23.degree. C was optimal for virus accumulation. Between 23-30.degree. C the rate at which lesions increased in diameter decreased with increasing temperature. The inhibitory effect of supraoptimal temperatures on the establishment of infection may be due to degradation of the infective entity by RNase(s).