External and internal acetylcholinesterase in rat sympathetic neurones in vivo and in vitro

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Abstract
The subcellular distribution of multiple molecular forms of acetylcholinesterase (AChE) in neurones of rat superior cervical ganglion (SCG) was determined both in vivo and in vitro by the use of selective lipidsoluble or -insoluble inhibitors. In vivo as well as in vitro, 10 S AChE is mainly outside the cell. In primary cultures of rat SCG neurones, both 4 S and 16 S AChE are mainly inside the cell. In near-term rat SCG, 4 S and 16 S are more external to the cell than in primary cultures. In adult rat SCG, 4 S AChE is equally distributed inside and outside and 16 S AChE is mainly outside the cell. Thus, specific AChE externalization probably occurs in neuronal cells as a developmentally regulated process