n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.
- 1 October 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (20) , 6128-6131
- https://doi.org/10.1073/pnas.79.20.6128
Abstract
The unusual NH2-terminal blocking group of the catalytic subunit of bovine cardiac muscle cAMP-dependent protein was found to be amide-linked n-tetradecanoic acid by gas chromatographic, direct chemical ionization and fast atom bombardment mass spectrometry. Fast atom bombardment mass spectrometry revealed the presence of an additional Ala which had been overlooked when the original sequence was determined. The corrected and completed NH2-terminal sequence of the 350-amino acid catalytic subunit is CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys.Keywords
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