Cloning and Expression of a Limonene Degradation Pathway from Bacillus stearothermophilus in Escherichia coli

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Abstract
The pathway encoding limonene catabolism was cloned as a 9.6kb chromosomal fragment from Bacillus stearothermophilus strain BR388 into Escherichia coli. It conferred growth on limonene as a sole carbon source and bioproduction of α‐terpineol, perillyl alcohol, and perillyl aldehyde. Incubation of the recombinant with perillyl alcohol resulted in formation of perillyl aldehyde and perillic acid. Catabolic pathways apparently affected the microbial toxicity of limonene.