Detection and identification of labeled DNA by surface enhanced resonance Raman scattering
- 1 January 2000
- journal article
- Published by Wiley in Biopolymers
- Vol. 57 (2) , 85-91
- https://doi.org/10.1002/(sici)1097-0282(2000)57:2<85::aid-bip5>3.0.co;2-#
Abstract
The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.Keywords
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