Reporter enzymes for the study of promoter activity
- 1 August 1994
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 2 (1) , 23-27
- https://doi.org/10.1007/bf02789287
Abstract
This article describes the use of three reporter enzymes used to study promoter activity in transgenic animals. Chloramphenicol acetyl transferase may be assayed by a nonchromatographic method that is rapid and sensitive. β-Galactosidase is measured by a photometric assay and luciferase is assayed by measuring the emission of light using a luminometer. The relative merits of each enzyme is discussed. Ths use of reporter enzymes provides a rapid and sensitive method for analysis of transgene expression.Keywords
This publication has 7 references indexed in Scilit:
- OPTIMIZED USE OF THE FIREFLY LUCIFERASE ASSAY AS A REPORTER GENE IN MAMMALIAN-CELL LINES1989
- Grafting genetically modified cells into the rat brain: characteristics of E. coli β-galactosidase as a reporter geneMolecular Brain Research, 1989
- Firefly luciferase luminescence assays using scintillation counters for quantitation in transfected mammalian cellsAnalytical Biochemistry, 1988
- Firefly luciferase gene: structure and expression in mammalian cells.Molecular and Cellular Biology, 1987
- In Situ Detection of β-Galactosidase in Lenses of Transgenic Mice with a γ-Crystallin/ lacZ GeneScience, 1987
- A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cellsAnalytical Biochemistry, 1986
- Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Molecular and Cellular Biology, 1982