Insulin receptor kinase in human skeletal muscle
- 1 July 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 186 (1) , 85-88
- https://doi.org/10.1016/0014-5793(85)81344-2
Abstract
Receptor‐associated protein kinase activity has been shown in all primary target tissues of insulin action in the rat and a function of insulin receptor phosphorylation in signal transmission was proposed. Insulin receptor phosphorylation so far has not been demonstrated in human target tissues of insulin. We describe here insulin receptor kinase activity in human skeletal muscle. Insulin (10−8 ) stimulates the phosphorylation of a 95‐kDa protein from skeletal muscle 2‐fold. The phosphoprotein is quantitatively immunoprecipitated with insulin receptor antibody identifying it as the β‐subunit of the insulin receptor. The insulin stimulation of phosphorylation is detectable also at physiological insulin concentrations (10−9 ) showing that receptor phosphorylation could be involved in insulin action in human skeletal muscle as well.Keywords
This publication has 16 references indexed in Scilit:
- Protein kinase activity of the insulin receptor from muscleFEBS Letters, 1984
- Insulin binding in human skeletal muscleBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Abnormality of insulin binding and receptor phosphorylation in an insulin-resistant melanoma cell line.The Journal of cell biology, 1984
- Phosphorylation and dephosphorylation of the insulin receptor: evidence against an intrinsic phosphatase activityBiochemistry, 1984
- Phosphorylation—dephosphorylation of purified insulin receptor from human placentaFEBS Letters, 1982
- Insulin activates a tyrosine-specific protein kinase in extracts of 3T3-L1 adipocytes and human placenta.Proceedings of the National Academy of Sciences, 1982
- Insulin receptor phosphorylation in intact adipocytes and in a cell-free systemBiochemical and Biophysical Research Communications, 1982
- Phosphorylation of the hepatic insulin receptorFEBS Letters, 1982
- Insulin Stimulates the Phosphorylation of the 95,000-Dalton Subunit of Its Own ReceptorScience, 1982
- Hexose transport in human adipocytes: Factors influencing the response to insulin and kinetics of methylglucose and glucose transportDiabetologia, 1981