Interference of fetal hemoglobin with the spectrophotometric measurement of carboxyhemoglobin.

30 April 1988

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 34 (5) , 975-977
https://doi.org/10.1093/clinchem/34.5.975

Abstract

We measured the concentration of carboxyhemoglobin (HbCO) in blood samples from 32 neonates by spectrophotometry (IL282 CO-Oximeter) and gas chromatography, finding a strong positive correlation (r = 0.89) between the concentration of fetal hemoglobin (Hb F) and HbCO as measured by spectrophotometry, but not by gas chromatography. Thus, Hb F interferes with the determination of HbCO by spectrophotometric techniques by falsely increasing apparent HbCO in direct proportion to Hb F. We conclude that, when Hb F is known or suspected to be present, blood HbCO cannot be reliably determined by methods based on spectrophotometry.

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