Tryptophan 54 and phenylalanine 60 are involved synergistically in the binding of E. coli SSB protein to single‐stranded polynucleotides
- 17 August 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 220 (2) , 347-352
- https://doi.org/10.1016/0014-5793(87)80844-x
Abstract
The binding of both wild‐type and point‐mutated E. coli single‐stranded DNA‐binding (SSB) protein to poly(deoxythymidylic acid) has been studied by fluorescence and optical detection of triplet state magnetic resonance spectroscopy. Involvement of tryptophan residues 40 and 54 in stacking interactions with nucleotide bases has been inferred earlier from such studies. Investigation of a point mutation in the E. coli SSB gene product obtained by site specific oligonucleotide mutagenesis in which Phe‐60 is replaced by alanine strongly suggests the participation of Phe‐60 in the binding process, possibly by the formation of an extended stacking structure by Trp‐54, thymine and Phe‐60. This hypothesis is supported by results on the point mutations in which His‐55 is replaced by either leucine or tyrosine.Keywords
This publication has 15 references indexed in Scilit:
- Role of tryptophan 54 in the binding of E. coli single‐stranded DNA‐binding protein to single‐stranded polynucleotidesFEBS Letters, 1987
- Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNABiochemistry, 1986
- SINGLE-STRANDED DNA BINDING PROTEINS REQUIRED FOR DNA REPLICATIONAnnual Review of Biochemistry, 1986
- Salt-dependent changes in the DNA binding co-operativity of Escherichia coli single strand binding proteinJournal of Molecular Biology, 1986
- Amplification of ssb-1 mutant single-stranded DNA-binding protein in Escherichia coliJournal of Molecular Biology, 1983
- Sequences of the ssb gene and protein.Proceedings of the National Academy of Sciences, 1981
- Amplification of single-strand DNA binding protein in Escherichis coliNucleic Acids Research, 1980
- Effect of stacking interactions with poly(riboadenylic acid) on the triplet state properties of tryptophanBiochemistry, 1978
- Structure of a Dinucleotide: Thymidylyl-(5′ → 3′)-Thymidylate-5′ (pTpT)Science, 1973
- Statistical thermodynamics of nucleic acid melting transitions with coupled binding equilibriaBiopolymers, 1971