Immunoglobulin Receptors on Mouse Mast Cells

Open Access
Publisher Website
Google Scholar
Abstract
Rosette formation around isolated mouse peritoneal mast cells by sheep erythrocytes coated with DNP37BSA and then reacted with various anti-DNP antibodies was used to investigate the types of antibody capable of binding to these mast cells. Mouse γG2 antibodies, obtained by electrophoretic fractionation, were found to have the ability to bind to mast cells. In addition, the rosette-forming capacity of mouse γG2-coated erythrocytes could be inhibited by the presence of γG1, γG2a, and γG2b purified mouse myeloma proteins, but not by γA of γM myelomas. These results directly demonstrate the existence of a class of antibody molecules which can bind to mast cells but which apparently cannot mediate the anaphylactic release of histamine from those cells. Of the heterologous antibodies studied, rat anti-DNP antibodies were the most effective in forming rosettes around mast cells: rabbit antibodies were much less effective. Guinea pig γG2 antibodies, which also are incapable of mediating anaphylactic reactions in the mouse, were nevertheless capable of binding to mouse mast cells. No receptors for complement could be detected on the surface of mouse peritoneal mast cells.

This publication has 0 references indexed in Scilit: