Improved rehydration by controlled drying of polyacrylamide gels for hybrid isoelectric focusing
- 1 January 1987
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 8 (12) , 584-585
- https://doi.org/10.1002/elps.1150081209
Abstract
A procedure is described to improve the properties of dried gels, containing immobilized pH gradients, for rehydration in the presence of urea. Triton X‐100, reducing agents and carrier ampholytes. When gels are dried by a fan in ambient atmosphere for the shortest time interval required to achieve an evenly flat surface, rehydration in the presence of 8 mol/L urea and 30 or 5 mL/L of Triton X‐100 is completed within 4 or 1 h, respectively. These rehydration properties can be maintained by storing the dried and sealed gels at −20 °C.Keywords
This publication has 12 references indexed in Scilit:
- Two‐dimensional electrophoresis with immobilized pH gradients in the first dimension: Protein focusing as a function of timeElectrophoresis, 1987
- Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergentElectrophoresis, 1987
- Avoiding liquid exudation on the surface of rehydrated gels used for hybrid isoelectric focusing in carrier ampholyte supplemented immobilized pH gradientsElectrophoresis, 1986
- High‐resolution two‐dimensional electrophoresis of myofibrillar proteins with immobilized pH gradientsElectrophoresis, 1985
- Isoelectric focusing and two-dimensional electrophoresis of tubulin using immobilized pH gradients under denaturing conditionsAnalytical Biochemistry, 1985
- Hybridisoelectric focusing in rehydrated immobilized pH gradients with added carrier ampholytes: Demonstration of human globinsElectrophoresis, 1985
- Improved horizontal two‐dimensional electrophoresis with hybrid isoelectric focusing in immobilized pH gradients in the first dimension and laying‐on transfer to the second dimensionElectrophoresis, 1985
- Membrane protein analysis by isoelectric focusing in immobilized pH gradientsElectrophoresis, 1985
- Swelling kinetics of immobiline gels for isoelectric focusingElectrophoresis, 1984
- Improved rehydration procedure for polyacrylamide gels in presence of urea: Demonstration of inherited human prealbumin variants by isoelectric focusing in an immobilized pH gradientElectrophoresis, 1984