Protamines in the sperm heads of inbred and outbred mice

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Abstract
The inbred strains used were C57, C3H, BALB, CBA and DBA. Outbred strains were obtained from a central animal breeding establishment where the breeding pattern was random and from a colony of wild mice obtained by trapping. Using polyacrylamide gel electrophoresis, it was found that the basis sperm proteins migrated with the polyarginine standard as a single peak and the amount of such protein in inbred animals was about 20% greater than in outbred animals. The clear-cut differences observed between inbred and outbread mouse spermatozoa, especially at neutral pH values of the solvent, suggest either that the sulfhydryl bonds in the sperm head protein of the inbred animal are less accessible to the reagent or that there are more of them. The latter explanation seems more likely, as indicated by the consistent finding of an increased amount of protamine in the polyacrylamide gel band prepared from an equivaleent number of spermatozoa from the inbred animals.

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