A physical method for separating Saccharomyces cerevisiae cells according to their ploidy
- 1 September 1988
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 34 (9) , 1102-1104
- https://doi.org/10.1139/m88-194
Abstract
A centrifugation technique, using genetically marked Saccharomyces cerevisiae strains, has been developed to separate Saccharomyces cerevisiae cells of different ploidy levels from exponential phase cultures. The method involves the conversion of yeast cells to protoplasts, the separation of the protoplasts on an osmotically stabilized Nycodenz gradient, and their regeneration. This type of selection may be of importance where selectable markers are not available.This publication has 2 references indexed in Scilit:
- Buoyant densities of macromolecules, macromolecular complexes, and cell organelles in Nycodenz gradientsAnalytical Biochemistry, 1983
- Formation of isotonic Nycodenz gradients for cell separationsAnalytical Biochemistry, 1982