Isolation of Organic Compounds from Water for Chemical Analysis and Toxicological Testing

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Abstract
In most investigations on water quality it is not possible to determine the relationship between toxicological and chemical parameters as a consequence of different isolation and sample preparation techniques. A combined research made by the analytical-chemical and biological section of KIWA led to the development of a single isolation and sample preparation method for organic micropollutants in water, suitable for toxicological and chemical measurements as the Ames test, group parameters (OCl, ON, OS, OP), GC-MS [gas chromatography-mass spectrometry] and HPLC [high performance liquid chromatography]. An automated installation for the adsorption of volatiles to Tenax and of slightly-volatile and non-volatile compounds at pH 7, 2 and 9 to XAD-4 was built. Following adsorption the volatiles were thermically desorbed from the Tenax. The XAD columns were eluted with ethanol and an ethanol-cyclohexane mixture. The eluates were dried and concentrated by an azeotropic distillation. This procedure was automated.