SYNTHESIS AND SECRETION OF ALPHA-1-MICROGLOBULIN BY HUMAN-LYMPHOCYTES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (1) , 134-139
Abstract
.alpha.1-Microglobulin (.alpha.1-m) was purified by column chromatography from a supernatant fluid of cultured T [thymus-derived] and B [bone marrow-derived] lymphocytes stimulated with mitogens. This protein had a MW of 33,000 as determined by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, migrated in the .alpha.1-region on immunoelectrophoresis and proved immunologically identical to .alpha.1-m which was purified from the urine of patients with renal tubular disorders. Using indirect immunofluorescence, .alpha.1-m was detected on the surface of T and B lymphocytes, displaying the same intensity on each cell type. .alpha.1-M is apparently actively produced and secreted by T and B lymphocytes.