Haematoxylin Stainable Epidermal Protein of the Newborn Rat

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Abstract
A haematoxylin stainable protein (HSP) extracted from the EDTA‐separated epidermis of 3‐day‐old rats was purified through preparative isoelectric focusing followed by fast protein liquid chromatography (FPLC) using a Superose 12 column and a Mono‐Q anion exchange column. The purified protein was a single protein in SDS‐PAGE; it had a molecular weight of 14,500 daltons and an isoelectric point of pH 4.7. The amino acid composition included 14% glutamic acid, 13.7% lysine, 12% aspartic acid, 10% glycine, 8% valine and 0.3% ornithine, but only 1.2% histidine.An antibody elicited by the purified antigen also localized in the cell membrane region of the stratum corneum cells of the flank skin, but not in the keratohyalin granules, in indirect immunofluorescent studies.

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