Purification and characterization of a labile rat glutathione transferase of the Mu class
- 15 June 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 260 (3) , 789-793
- https://doi.org/10.1042/bj2600789
Abstract
A labile GSH transferase homodimer termed 11-11 was purfied from rat testis by GSH-agarose affinity chromatography followed by aninon-exchange f.p.l.c. The enzyme is unstable in the absence of thiol(s) and has relatively low affinity for both 1-chloro-2,4,-dinitrobenzene (Km 4.4 mM) and GSH (Km(app.) 4.4 mM). Its mobility on SDS/polyacrylamide-gel electrophoresis is slightly less than that of subunits 3 and 4 and its pI is 5.2. Subunit 11 has a blocked N-terminal amino acid residue, but after CNBr cleavage fragments accounting for 113 amino acid residues were sequenced and showed 65% homology with corresponding sequences in subunit 4, indicating that it is a member of the Mu family. GSH transferase 11 is a major isoenzyme in testis, epididymis, prostate and brain and present at lower concentrations in other tissues.This publication has 29 references indexed in Scilit:
- Selective expression of a unique glutathione S-transferase Yb3 gene in rat brain.Journal of Biological Chemistry, 1987
- Enzymatic transformation of PGH2 to PGF2α catalyzed by glutathione S-transferasesBiochemical and Biophysical Research Communications, 1986
- Rat glutathione transferase 8‐8, an enzyme efficiently detoxifying 4‐hydroxyalk‐2‐enalsFEBS Letters, 1986
- DIFFERENCES IN STEREOSELECTIVITY AND CATALYTIC EFFICIENCY OF 3 HUMAN GLUTATHIONE TRANSFERASES IN THE CONJUGATION OF GLUTATHIONE WITH 7-BETA,8-ALPHA-DIHYDROXY-9-ALPHA,10-ALPHA-OXY-7,8,9,10-TETRAHYDROBENZO(A)PYRENE1986
- The enzymatic conjugation of glutathione with bay-region diol-epoxides of benzo[a]pyrene, benz[a]anthracene and chryseneCarcinogenesis: Integrative Cancer Research, 1986
- Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties.Proceedings of the National Academy of Sciences, 1985
- Isolation and characterization of the multiple glutathione S-transferases from human liver. Evidence for unique heme-binding sites.Journal of Biological Chemistry, 1985
- Glutathione transferases in primary rat hepatomas: the isolation of a form with GSH peroxidase activityFEBS Letters, 1985
- Glutathione transferases: NomenclatureBiochemical Pharmacology, 1984
- Inhibition of microsomal lipid peroxidation by glutathione and glutathione transferases B and AA. Role of endogenous phospholipase A2Biochemical Journal, 1984