A 23 911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the Iev operon
- 1 April 1997
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 143 (4) , 1321-1326
- https://doi.org/10.1099/00221287-143-4-1321
Abstract
Within the framework of the European project to sequence the whole Bacillus subtilis 168 genome, a 23 911 bp long chromosomal DNA fragment located around 233° on the B. subtilis genetic map was cloned and sequenced. From the generated sequencing data and the results of the homology search, the primary structure of this region was determined. In addition to the whole Iev operon, the region contains putative genes for an amino acid permease, two different alcohol dehydrogenases, a chitosanase, a protein belonging to the LysR family of transcriptional regulators, a protein related to the MerR transcriptional regulator, up to four proteins related to the product of the spoF gene, and genes coding for nine more inferred proteins of unknown function.Keywords
This publication has 20 references indexed in Scilit:
- Mapping of the 150 kb spoIIIC-pheA region of the Bacillus subtilis chromosome using Long Accurate PCR and three yeast artificial chromosomesMicrobiology, 1996
- Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae RdScience, 1995
- Analysis of theEscherichia coligenome VI: DNA sequence of the region from 92.8 through 100 minutesNucleic Acids Research, 1995
- Complete mitochondrial genome amplificationNature Genetics, 1994
- PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.Proceedings of the National Academy of Sciences, 1994
- Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325° to 333deg;Molecular Microbiology, 1993
- The major piscine liver alcohol dehydrogenase has class-mixed properties in relation to mammalian alcohol dehydrogenases of classes I and IIIBiochemistry, 1992
- A sequence assembly and editing program for efficient management of large projectsNucleic Acids Research, 1991
- Gene encoding two alkali-soluble components of the spore coat from Bacillus subtilisJournal of Bacteriology, 1991
- The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators.Proceedings of the National Academy of Sciences, 1991