Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2and HPLC purification of synthetic RNA
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (19) , 5131-5136
- https://doi.org/10.1093/nar/20.19.5131
Abstract
The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-2H]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60 degrees C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purification method has been effectively applied to other RNA sequences and will assist biophysical studies which require relatively large quantities of RNA oligomers.Keywords
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