Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle
Open Access
- 15 July 1984
- journal article
- Published by American Veterinary Medical Association (AVMA) in Journal of the American Veterinary Medical Association
- Vol. 185 (2) , 179-182
- https://doi.org/10.2460/javma.1984.185.02.179
Abstract
SUMMARY: During a 19-month period, the agar gel immunodiffusion (agid) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were agid test-positive for paratuberculosis. Twenty-eight cattle (87.5%) yielded Mycobacterium paratuberculosis from bacterial culture of feces. Four cattle were confirmed as having paratuberculosis on necropsy. One had a negative fecal culture but was lost to follow-up. Thus, 32 of the 33 agid test-positive cattle (96.9%) were confirmed as paratuberculous by culture or necropsy. Of the 18 cattle that were agid test-negative, 17 (94%) also were culture-negative after 12 to 20 weeks’ incubation of the culture tubes. Direct fecal smears from 29 confirmed paratuberculous cattle were examined. Twenty-three (79.3%) had smears positive for M paratuberculosis. Fifteen culture-negative cattle were examined by direct smear; the results were negative for all.This publication has 0 references indexed in Scilit: