Thin-Layer Chromatographic Screening and Confirmation of Methadone and Its Primary Metabolite in Urine
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Clinical Toxicology
- Vol. 16 (1) , 55-59
- https://doi.org/10.3109/15563658008989924
Abstract
Methadone [itself a drug of abuse] is extensively used in maintenance and detoxification programs for the treatment of heroin addiction. A TLC method for the simultaneous screening and confirmation of methadone and its primary metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine) in human urine specimens is presented. Urine is made basic and extracted with organic solvent. After separation and concentration, the residue is spotted, half on each of 2 TLC plates. One plate is developed in an ethyl acetate:methanol:diethylamine (90:10:1.6) solvent system and the 2nd in an ethyl acetate:methylene chloride:propylamine (85:17:1.0) solvent system. Methadone and its primary metabolite can be detected in concentrations as low as 0.5 .mu.g/ml without interference from other drugs and urinary substances.This publication has 4 references indexed in Scilit:
- A Comparison of Methods used in the Detection of Methadone and its Primary MetaboliteJournal of Analytical Toxicology, 1977
- GLC Determination of Methadone in Human UrineJournal of Pharmaceutical Sciences, 1972
- A gas-liquid chromatographic method for the quantitative determination of methadone in human plasma and urine.Journal of Chromatography A, 1972
- Urinary excretion of methadone in manClinical Pharmacology & Therapeutics, 1972