Resonance Raman Spectra of Riboflavin and Its Derivatives in the Bound State with Egg Riboflavin Binding Proteins

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Abstract
The resonance Raman spectra of riboflavin (RF) and its derivatives, including 3-deuterated (3-D RF), 3-methyl (3-CH3 RF), 3-carboxymethyl (3-CH2COOH RF), and 7,8-dichloro riboflavins (7,8-Cl RF), in H2O and D2O were observed in the 700–1700 cm−1 region. The fluorescence problem of riboflavin was overcome by complex formation of riboflavin with riboflavin binding proteins. The observed frequencies of Raman lines of RF are in good agreement with those of glucose oxidase obtained by Spiro et al. by the resonance CARS method, although the present spectral range is extended to much lower frequency with a higher signal-to-noise ratio than that for glucose oxidase. The observed Raman lines were assigned to the individual ring modes of isoalloxazine on the basis of the Raman spectra of appropriate model compounds such as uracil, pyrazine, and o-xylene. The 1253 cm−1 line of RF was shifted to ca. 1300 cm−1 for 3-D RF, 3-CH3 RF, and 3-CH2COOH RF, and accordingly can be assigned to the CN stretching mode of Ring III. The 1632 cm−1 line of RF was shifted for 7,8-C1 RF and was assigned to a Ring I mode. No Raman line mainly due to C=O stretching mode was observed in the present resonance Raman spectra.

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