Expression of Two Kinds of Cytochrome P-450(11β) mRNA in Bovine Adrenal Cortex
- 1 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 104 (5) , 683-686
- https://doi.org/10.1093/oxfordjournals.jbchem.a122533
Abstract
Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559–568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.This publication has 23 references indexed in Scilit:
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