Detection and incidence of specific species of spoilage bacteria on fish. I. Methodology.

  • 1 November 1968
    • journal article
    • Vol. 16  (11) , 1734-7
Abstract
The ability of Pseudomonas putrefaciens to form H(2)S was found to serve as a singularly useful criterion of identity for this species and was used to directly enumerate the organism from haddock fillets by the use of pour plates of Peptone-Iron Agar. Subsurface colonies appear intensely black, whereas surface colonies are black or gray. A highly sensitive soft-agar-gelatin overlay technique has been found useful for directly determining the numbers of weakly and strongly proteolytic organisms from fish tissue.

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