INDUCTION OF ANCHORAGE-INDEPENDENT GROWTH IN HUMAN-FIBROBLASTS BY PROPANE SULTONE
- 1 January 1981
- journal article
- research article
- Vol. 41 (5) , 1620-1627
Abstract
A dose-dependent increase in the frequency of diploid human cells capable of anchorage-independent (Al) growth after treatment with the carcinogen propane sultone, followed by exponential growth to allow full expression of this phenotype (8-13 population doublings). Exposure to these same concentrations of propane sultone also resulted in a dose-dependent increase in the frequency of 6-thioguanine-resistant cells in the population. Procedures such as synchronization of cells and treatment just after the onset of DNA synthesis or the use of special selective medium were not essential for this induction. A very low frequency of cells with the Al phenotype was found in the control population (background). Cells which exhibited the Al phenotype spontaneously or after carcinogen treatment retained the characteristic over as many generations as tested (> 13). Al growth apparently is the result of a mutational event.This publication has 3 references indexed in Scilit:
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- Evidence for the progressive nature of neoplastic transformation in vitro.Proceedings of the National Academy of Sciences, 1978
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