Characterization of plant coumarins by combined gas chromatography, ultraviolet absorption spectroscopy, and nuclear magnetic resonance analysis
- 1 October 1969
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Chemistry
- Vol. 47 (19) , 3577-3583
- https://doi.org/10.1139/v69-591
Abstract
A method for the characterization of sub-milligram quantities of plant coumarins has been devised. Each compound was purified by gas–liquid chromatography, and the collected material analyzed by a combination of ultraviolet absorption and nuclear magnetic resonance spectroscopy. The procedures developed were tested successfully on several plant extracts. Limettin and bergapten were identified in lime peel; angelicin, psoralen, and xanthotoxin in parsnips; and angelicin, xanthotoxin, and bergapten in Angelica leaves. Isopimpinellin was found in all three plant materials.Keywords
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